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dr feng zhang  (Addgene inc)


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    Structured Review

    Addgene inc dr feng zhang
    Dr Feng Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 6191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dr+feng+zhang/lentiCRISPR+v2+(Plasmid+%2352961)/pm41770818-501-16-19
    Average 96 stars, based on 6191 article reviews
    dr feng zhang - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
    Article Snippet: .. For this, the guide RNAs (gRNAs) that appear below were cloned into LentiCRISPRv2, a gift from Dr. Feng Zhang (Addgene plasmid# 52961) [109], using the BsmBI unique restriction site. ..

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    Article Title: ENO1 Dysfunction-Mediated Glycolytic Attenuation Exacerbates Oxidative Stress-Induced Retinal Ganglion Cell Death via Altered ATP Synthesis Pathway
    Article Snippet: .. Double-stranded oligonucleotides corresponding to sgENO1(209fw), sgENO2(50rv), and sgENO1(85rv) were ligated into the CRISPR-Cas9 plasmid (px601), provided by Dr. Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID: Addgene_61591), generating px601-sgENO1(209fw), px601-sgENO2(50rv), and px601-sgENO1(85rv).1 To construct gain-of-function plasmids, mouse ENO1 (NM_001379129) and mouse ENO2 (NM_013509.3) sequences were amplified by reverse transcription PCR from total RNA extracted from mouse retinas and cloned into pcDNA3-myc vectors (pcDNA3-ENO1-myc and pcDNA3-ENO2-myc, respectively). ..

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells.
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    Article Title: HIV-1 Vpr is targeted for degradation by autophagy
    Article Snippet: .. For this, the guide RNAs (gRNAs) that appear below were cloned into LentiCRISPRv2, a gift from Dr. Feng Zhang (Addgene plasmid# 52961) [ ], using the BsmBI unique restriction site. ..

    Plasmid Preparation:

    Article Title: HIV-1 Vpr is targeted for degradation by autophagy.
    Article Snippet: .. For this, the guide RNAs (gRNAs) that appear below were cloned into LentiCRISPRv2, a gift from Dr. Feng Zhang (Addgene plasmid# 52961) [109], using the BsmBI unique restriction site. ..

    Article Title: Culture condition-dependent cytotoxicity of bendamustine in mouse embryonic stem cells, independent of STAT3 and p53 pathways.
    Article Snippet: Bendamustine, an alkylating agent with STAT3-inhibitory activity in cancer cells, was investigated using mouse embryonic stem (ES) cells as a model. ES cells are valuable for understanding drug studies owing to their selfrenewal and pluripotency under defined culture conditions.. This study uncovered novel, STAT3-and p53-independent cytotoxic mechanisms of bendamustine using mouse ES cells as a model system.. Although it acts primarily through STAT3 inhibition in cancer cells, bendamustine induced potent cytotoxicity in ES cells without affecting STAT3 signaling, demonstrated by maintained STAT3 phosphorylation, transcriptional activity, and target gene expression.

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    Article Title: ENO1 Dysfunction-Mediated Glycolytic Attenuation Exacerbates Oxidative Stress-Induced Retinal Ganglion Cell Death via Altered ATP Synthesis Pathway
    Article Snippet: .. Double-stranded oligonucleotides corresponding to sgENO1(209fw), sgENO2(50rv), and sgENO1(85rv) were ligated into the CRISPR-Cas9 plasmid (px601), provided by Dr. Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID: Addgene_61591), generating px601-sgENO1(209fw), px601-sgENO2(50rv), and px601-sgENO1(85rv).1 To construct gain-of-function plasmids, mouse ENO1 (NM_001379129) and mouse ENO2 (NM_013509.3) sequences were amplified by reverse transcription PCR from total RNA extracted from mouse retinas and cloned into pcDNA3-myc vectors (pcDNA3-ENO1-myc and pcDNA3-ENO2-myc, respectively). ..

    Article Title: Atomic mechanisms of full-length ASC-mediated inflammasome assembly
    Article Snippet: .. The lentiCRISPRv2 plasmid, a kind gift from Dr. Feng Zhang (Addgene plasmid # 52961), was used to express Cas9 and guide RNA (gRNA). ..

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells.
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    Article Title: Secreted Protein Production is Improved by Controlling Endoplasmic Reticulum Stress Associated Protein Degradation
    Article Snippet: .. MDA-MB-231 IRE1α knockout cells were generated using CRISPR-Cas9 knockout with plasmids gifted by Dr. Feng Zhang (Addgene plasmid # 48138 and # 62988) and Dr. Andrea Ventura (Addgene plasmid # 64073). ..

    Article Title: HIV-1 Vpr is targeted for degradation by autophagy
    Article Snippet: .. For this, the guide RNAs (gRNAs) that appear below were cloned into LentiCRISPRv2, a gift from Dr. Feng Zhang (Addgene plasmid# 52961) [ ], using the BsmBI unique restriction site. ..

    Construct:

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    Article Title: ENO1 Dysfunction-Mediated Glycolytic Attenuation Exacerbates Oxidative Stress-Induced Retinal Ganglion Cell Death via Altered ATP Synthesis Pathway
    Article Snippet: .. Double-stranded oligonucleotides corresponding to sgENO1(209fw), sgENO2(50rv), and sgENO1(85rv) were ligated into the CRISPR-Cas9 plasmid (px601), provided by Dr. Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID: Addgene_61591), generating px601-sgENO1(209fw), px601-sgENO2(50rv), and px601-sgENO1(85rv).1 To construct gain-of-function plasmids, mouse ENO1 (NM_001379129) and mouse ENO2 (NM_013509.3) sequences were amplified by reverse transcription PCR from total RNA extracted from mouse retinas and cloned into pcDNA3-myc vectors (pcDNA3-ENO1-myc and pcDNA3-ENO2-myc, respectively). ..

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells.
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    Sequencing:

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    Article Title: Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cells.
    Article Snippet: .. pLentiCRISPR v2 was from Dr. Feng Zhang (Addgene plasmid #52961). pLentiCRISPR v2 sgPLIN3 was constructed by ligating an oligonucleotide duplex (Integrated DNA Technologies) into the BsmBI-v2 site (R0739S, New England Biolabs). pcDNA3 Flag PLIN3 was cloned by synthesizing PLIN3 cDNA sequence (GenScript) into the Nhe I and Xho I sites of pcDNA3 (Invitrogen). .. PLIN3 1219C > T mutant was generated from pcDNA3 Flag PLIN3 using the QuikChange II Site-Directed Mutagenesis Kit (200523, Agilent), following the manufacturer’s instructions.

    CRISPR:

    Article Title: ENO1 Dysfunction-Mediated Glycolytic Attenuation Exacerbates Oxidative Stress-Induced Retinal Ganglion Cell Death via Altered ATP Synthesis Pathway
    Article Snippet: .. Double-stranded oligonucleotides corresponding to sgENO1(209fw), sgENO2(50rv), and sgENO1(85rv) were ligated into the CRISPR-Cas9 plasmid (px601), provided by Dr. Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID: Addgene_61591), generating px601-sgENO1(209fw), px601-sgENO2(50rv), and px601-sgENO1(85rv).1 To construct gain-of-function plasmids, mouse ENO1 (NM_001379129) and mouse ENO2 (NM_013509.3) sequences were amplified by reverse transcription PCR from total RNA extracted from mouse retinas and cloned into pcDNA3-myc vectors (pcDNA3-ENO1-myc and pcDNA3-ENO2-myc, respectively). ..

    Article Title: Secreted Protein Production is Improved by Controlling Endoplasmic Reticulum Stress Associated Protein Degradation
    Article Snippet: .. MDA-MB-231 IRE1α knockout cells were generated using CRISPR-Cas9 knockout with plasmids gifted by Dr. Feng Zhang (Addgene plasmid # 48138 and # 62988) and Dr. Andrea Ventura (Addgene plasmid # 64073). ..

    Amplification:

    Article Title: ENO1 Dysfunction-Mediated Glycolytic Attenuation Exacerbates Oxidative Stress-Induced Retinal Ganglion Cell Death via Altered ATP Synthesis Pathway
    Article Snippet: .. Double-stranded oligonucleotides corresponding to sgENO1(209fw), sgENO2(50rv), and sgENO1(85rv) were ligated into the CRISPR-Cas9 plasmid (px601), provided by Dr. Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID: Addgene_61591), generating px601-sgENO1(209fw), px601-sgENO2(50rv), and px601-sgENO1(85rv).1 To construct gain-of-function plasmids, mouse ENO1 (NM_001379129) and mouse ENO2 (NM_013509.3) sequences were amplified by reverse transcription PCR from total RNA extracted from mouse retinas and cloned into pcDNA3-myc vectors (pcDNA3-ENO1-myc and pcDNA3-ENO2-myc, respectively). ..

    Reverse Transcription:

    Article Title: ENO1 Dysfunction-Mediated Glycolytic Attenuation Exacerbates Oxidative Stress-Induced Retinal Ganglion Cell Death via Altered ATP Synthesis Pathway
    Article Snippet: .. Double-stranded oligonucleotides corresponding to sgENO1(209fw), sgENO2(50rv), and sgENO1(85rv) were ligated into the CRISPR-Cas9 plasmid (px601), provided by Dr. Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID: Addgene_61591), generating px601-sgENO1(209fw), px601-sgENO2(50rv), and px601-sgENO1(85rv).1 To construct gain-of-function plasmids, mouse ENO1 (NM_001379129) and mouse ENO2 (NM_013509.3) sequences were amplified by reverse transcription PCR from total RNA extracted from mouse retinas and cloned into pcDNA3-myc vectors (pcDNA3-ENO1-myc and pcDNA3-ENO2-myc, respectively). ..

    Polymerase Chain Reaction:

    Article Title: ENO1 Dysfunction-Mediated Glycolytic Attenuation Exacerbates Oxidative Stress-Induced Retinal Ganglion Cell Death via Altered ATP Synthesis Pathway
    Article Snippet: .. Double-stranded oligonucleotides corresponding to sgENO1(209fw), sgENO2(50rv), and sgENO1(85rv) were ligated into the CRISPR-Cas9 plasmid (px601), provided by Dr. Feng Zhang (Addgene plasmid #61591; http://n2t.net/addgene:61591; RRID: Addgene_61591), generating px601-sgENO1(209fw), px601-sgENO2(50rv), and px601-sgENO1(85rv).1 To construct gain-of-function plasmids, mouse ENO1 (NM_001379129) and mouse ENO2 (NM_013509.3) sequences were amplified by reverse transcription PCR from total RNA extracted from mouse retinas and cloned into pcDNA3-myc vectors (pcDNA3-ENO1-myc and pcDNA3-ENO2-myc, respectively). ..

    Multiple Displacement Amplification:

    Article Title: Secreted Protein Production is Improved by Controlling Endoplasmic Reticulum Stress Associated Protein Degradation
    Article Snippet: .. MDA-MB-231 IRE1α knockout cells were generated using CRISPR-Cas9 knockout with plasmids gifted by Dr. Feng Zhang (Addgene plasmid # 48138 and # 62988) and Dr. Andrea Ventura (Addgene plasmid # 64073). ..

    Knock-Out:

    Article Title: Secreted Protein Production is Improved by Controlling Endoplasmic Reticulum Stress Associated Protein Degradation
    Article Snippet: .. MDA-MB-231 IRE1α knockout cells were generated using CRISPR-Cas9 knockout with plasmids gifted by Dr. Feng Zhang (Addgene plasmid # 48138 and # 62988) and Dr. Andrea Ventura (Addgene plasmid # 64073). ..

    Generated:

    Article Title: Secreted Protein Production is Improved by Controlling Endoplasmic Reticulum Stress Associated Protein Degradation
    Article Snippet: .. MDA-MB-231 IRE1α knockout cells were generated using CRISPR-Cas9 knockout with plasmids gifted by Dr. Feng Zhang (Addgene plasmid # 48138 and # 62988) and Dr. Andrea Ventura (Addgene plasmid # 64073). ..



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